Morphological characterisation and antimicrobial resistance of bacterial isolates from bovine mastitis

The antimicrobial susceptibility of the pathogens is essential for successful treatment bovine mastitis. Antimicrobial resistance develops in microbes as a result indiscriminate use antibiotics. Therefore, regular screening required selection appropriate and effective antimicrobials. A study was carried out 60 crossbred dairy cows early lactation with objective to assess morphological characteristics bacterial isolates from California mastitis test (CMT) done 235 individual quarter milk samples. Isolation bacteria by direct streaking CMT positive samples on blood agar followed incubation plates at 37°C 24 h. On Gram's staining, 32 were found be Gram cocci, three bacilli ten negative bacilli. disc diffusion assay revealed that highest against tetracycline (71.11 per cent), ampicillin ceftriaxone-tazobactam (66.67 ceftriaxone (64.44 amoxicillin-sulbactam (62.22 amikacin (57.78 ceftriaxone-sulbactam (55.56 enrofloxacin (46.67 amoxicillin-clavulanate (40 ciprofloxacin (37.78 cent) gentamicin co-trimoxazole (28.89 cent).